Tourism is one of the fastest growing sectors in the global economy. Tourism is one of the major sources of revenues in leading countries annually compared to other economic activities. In fact, tourism is the world's greatest industry, which is growing day after day. Iran has great potentials to attract tourists; however, this industry is not well developed unfortunately. Using effective marketing tools and parameters can help to develop and improve tourism industry in Iran. According to theoretical foundations of this research and consultation with ecotourism experts, four variables including building culture, educational level, environment protection and familiarity with nature were considered as independent variable while ecotourism development was considered as dependent variable in this study. The relationship and effect of independent variables on the dependent variable was investigated in this study. Descriptive research method was used to conduct the study. This is an applied research considering the goal of the research. The statistical population included managers and experts in the field of ecotourism in following selected cities of Gilan province including Anzali, Lahijan, Masal, Fooman, Rasht, Langrood, Roudsar, Roudbar, Talesh and Astara in this study. The statistical population was infinite. The samples were selected using random sampling method. The sample size was obtained as 82. To ensure that all questionnaires were filled out, 100 questionnaires were given to the sample. In this study, 10 cities from 16 cities of Gilan province were selected, which were more populous. The questionnaires were distributed among them. In order to obtain solutions for the research issue, four components were developed in a conceptual model. Several questions were designed for each one of components. Finally, a questionnaire with twenty-two questions were designed and given to individuals within the statistical population. After the questionnaires were filled out, the obtained findings were analyzed using SPSS software. Finally, it was concluded that all four components have a positive impact on ecotourism industry. These components include building culture by ecotourism organizations, familiarity with nature, awareness of the people and eco-tourism travel guide in the field of ecotourism, educational level in order to create a positive attitude in the staff and people toward eco-tourism travel guide in the field of ecotourism and environmental protection by local people and eco-tourism travel guide in Gilan province.